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Cell Selection and Reporter System
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A fusion protein has three domains, being an extracellular cell surface antigen or a fluorescent protein for sorting uses, an enzyme that enables staining of cells in tissues or tissue sections and an enzyme, such as antibiotic resistance, which protects cells from the effects of a toxic factor in culture medium. The fusion is used for purification and sorting of cells expressing the fusion under control of a cell or tissue-specific promoter and subsequent tracking and analysis of cells post transplantation.
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Reprogramming and Genetic Modification of Cells
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Methods for reprogramming and optional genetic modification of cells are provided. A pluripotent genome is obtained from a differentiated genome by fusing a pluripotent cell with a differentiated cell in the presence of Nanog or a MEK inhibitor. A cell is genetically modified by providing first and second cells, each containing chromosomes, fusing the first cell and the second cell, and culturing the fused cell so as to obtain a diploid cell containing at least one chromosome from the first cell and at least one chromosome form the second cell. A method of cell fusion comprises fusing a first cell and a second cell in the presence of Nanog or a MEK inhibitor. Cells obtained thereby and their uses are also described.
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